Fig. 4
From: Tissue engineered corneal endothelium transplantation in an ex vivo human cornea organ culture model
Immunofluorescence and cross-sectional H&E staining following 14 days of RAFT transplantation. (A, B) There were no cells on the acellular RAFT graft and no cells expressing ZO-1 and Na/K ATP on the cell–cell borders. (C) The cross-sectional H&E staining showed that an acellular RAFT graft (❖) was attached to the posterior surface of the cornea, and no cells grew on the graft. (D, E) Cells on the cell-seeded-RAFT graft expressed Na/K ATPase on the cell–cell borders, retaining close cell–cell contact with a high cell density. (F) A monolayer of endothelial cells (▲) was observed on the surface of the cell-seeded-RAFT graft (✱). Images are representative of n = 3 biological replicates. Scale bar = 50 µm for the immunostaining images. Scale bar = 100 µm for the cross-sectional H&E staining images.
