Fig. 1

CENP-F forms temporally regulated PLA ligation products with known interactors at mitotic kinetochores in RPE-1 cells. (a) The BUB1/CENP-F pair forms PLA products that are particularly abundant in prometaphase and concentrate in the region of microtubule-chromosome contacts. (b) NUP133 and CENP-F form PLA products that peak during chromosome alignment in metaphase. Scale bar: 10 µm.