Fig. 2 | Scientific Reports

Fig. 2

From: Dynamic monitoring of M-protein quantification by immunotyping using capillary zone electrophoresis during the chemotherapy of patients with multiple myeloma

Fig. 2

Comparison of the results of immunofixation electrophoresis and immunotyping in the β region of M-protein (IgAλ). All three subfigures were derived from the same patient, a 77-year-old male, found during an inpatient examination in the Department of Nephrology. (A) Immunofixation electrophoresis (IFE). In the electrophoresis (ELP) lane, the M-protein is indistinguishable from the normal protein in the β zone; in addition, the color intensity in the antibody lane is higher than the same position in the ELP lane (for example, the color intensity is significantly higher in the IgG lane than that in the γ zone of the ELP lane). (B) Immunotyping using immunosubtraction. The electrophoresis pattern after adding antibodies was compared with that of ELP, and the position of M-protein in ELP can be distinguished. (C) Capillary zone electrophoresis, with the position of the M-protein identified in B marked by a tangent line.

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