Fig. 5
From: Cystatin 6 (CST6) and Legumain (LGMN) are potential mediators in the pathogenesis of preeclampsia
Single cell RNA sequencing analysis of CST6 and LGMN in hTSC organoids. Transcriptomic analysis of a publicly available scRNA-seq dataset of three-dimensional hTSC-derived organoids (n = 3) treated under hTSC conditions or induced to differentiate to EVTs across 21 days (GEO accession number GSE17448127). Analysis resulted in 6 distinct transcriptomic cell identities: cytotrophoblast (CTB), proliferative cytotrophoblast (CTBprol), pre-fusion cytotrophoblast (CTBpf), progenitor EVT (pEVT), invasive EVT (iEVT) and syncytiotrophoblast (STB). UMAP plot of cell identities in (A) undifferentiated and (B) EVT differentiated hTSC organoids. (C) UMAP plot of CST6 and LGMN expression relative to cell identities in undifferentiated organoids. (D) UMAP plot of CST6 and LGMN expression relative to cell identities in EVT differentiated organoids. (E) Dot plot of CST6 and LGMN to indicate levels of gene expression in cell proportions of specific cell identities for each condition.
