Fig. 6
From: Cystatin 6 (CST6) and Legumain (LGMN) are potential mediators in the pathogenesis of preeclampsia
Single-nuclei RNA sequencing (snRNA-seq) analysis of early-onset preeclamptic, healthy and preterm placenta. (A) and (B), Uniform manifold approximation and projection (UMAP) visualising and analysis resulted in 7 distinct transcriptomic cell identities obtained from the data: villous syncytiotrophoblast (vSTB1 and vSTB2), villous juvenile syncytiotrophoblast (vSTBjuv), villous pre-fusion cytotrophoblast (vCTBpf), villous proliferative cytotrophoblast (vCTBp), villous cytotrophoblast (vCTB) and villous cell column trophoblast (vCCT). Samples from preterm pregnancy, early-onset preeclampsia and term healthy pregnancy were integrated for visualisation. Each dot represents a nucleus with each colour representing a cell identity. (C) Violin plot of CST6 and LGMN to indicate levels of gene expression in cell proportions of specific cell identities for each condition. Code available at https://github.com/HiDiHlabs/preeclamspsia_Nonn_etal/, data available in supplementary methods of manuscript28.
