Fig. 9
From: Cystatin 6 (CST6) and Legumain (LGMN) are potential mediators in the pathogenesis of preeclampsia
Recombinant CST6 enhances induction of endothelial dysfunction and LGMN in HUVECs. Induction of endothelial dysfunction in HUVECs was assessed by induction of markers: ICAM1 (A), VCAM1 (B) and ET-1 (C). Recombinant CST6 enhanced induction of dysfunction markers and (D) LGMN in HUVECs under inflammatory conditions. mRNA expression was normalised to the appropriate housekeeping gene. To calculate significance, one-way ANOVA (parametric) or a Kruskal Wallis test (non-parametric) was used. Data expressed as mean ± SEM for experiments (A, C and D) or median ± IQR (B). Experiments repeated n = 5 in duplicate. *** p < 0.001, * p < 0.05.
