Table 1 Validation results summary.

From: Detection and accurate identification of Mycobacterium species by flow injection tandem mass spectrometry (FIA-MS/MS) analysis of mycolic acids

Parameter

M. tuberculosis H37Rv ATCC 25618

M. smegmatis

ATCC 19420

M. absessus

ATCC 23045

Limit of detection (S/N)

0,6*101 CFU (4)

0,2*101 CFU (5)

0,4*101 CFU (4)

Lower limit of quantitation (S/N)

1,6*101 CFU (18)

1,0*101 CFU (20)

1,3*101 CFU (18)

Upper limit of quantitation (R2)

5,7*107 CFU (0,999)

1,8*107 CFU (0,997)

3,2*107 CFU (0,995)

Precision

± 1,2%

± 2,9%

± 2,2%

Repeatability

± 2,1%

± 4,5%

± 3,8%

Reproducibility

± 9,3%

± 15,3%

± 12,5%