Fig. 2 | Scientific Reports

Fig. 2

From: Involvement of circadian clock protein PER2 in controlling sleep deprivation induced HMGB1 up-regulation by targeting p300 in the cortex

Fig. 2

Sleep deprivation induced acetylation-dependent nuclear export of HMGB1 in the cortex. (A) Rats were subjected to sleep deprivation for 72 h, and then the expression levels of cortical HMGB1 mRNA before and after sleep deprivation were detected with RT-PCR assay. (N = 3) (B)Relative expression and quantification of the mRNA in Fig. 2A were shown. (C) Rats were subjected to sleep deprivation for 72 h, and then cortex tissue lysate was subjected to immunoprecipitation with the anti-HMGB1 antibody followed by blotting with anti-acetyl-lysine antibody. (N = 3) (D) Rats were subjected to sleep deprivation for 72 h, and then the cytoplasmic/nuclear distribution of HMGB1 in the cortex tissue lysate before and after sleep deprivation were detected. (N = 3) (E) Relative expression and quantification of the proteins in Fig. 2D were shown. Data are expressed as the mean ± SD. NS: no significance, * p < 0.05, **p < 0.01.

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