Fig. 5 | Scientific Reports

Fig. 5

From: Involvement of circadian clock protein PER2 in controlling sleep deprivation induced HMGB1 up-regulation by targeting p300 in the cortex

Fig. 5

PER2 acted as a co-suppressor of p300 in microglial cells. (A) BV2 cells were transfected with FLAG-PER2 expression plasmid or its control vector, and then the whole cell lysate were immunorepcipitated with anti-FLAG antibody followed by blotting with anti-p300 antibody to detect the potential interaction of PER2 and p300. (B) Design of the truncated PER2 mutants. (C) BV2 cells were transfected with WT-PER2 or the truncated PER2 mutants, and then the whole cell lysate were immunorepcipitated with anti-FLAG antibody followed by blotting with anti-p300 antibody to detect p300 binding domain within PER2. (D) BV2 cells were transfected with PER2 siRNA or its control siRNA, followed by reconstitution with WT-PER2 or the truncated PER2 mutants. Then the expression levels of p300 were detected at 36 h later.

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