Fig. 5 | Scientific Reports

Fig. 5

From: Oroxylin A may promote cell apoptosis and inhibit epithelial-mesenchymal transition in endometrial cancer, associated with the ERβ/PI3K/AKT pathway

Fig. 5The alternative text for this image may have been generated using AI.

OA exerted anti-EC effects by regulating the ERβ/PI3K/AKT pathway. (A) ERβ mRNA expression level. (B) Protein expression levels of ERβ, AKT, p-AKT(Ser473) and PI3K. (C) Relative protein expression of ERβ. (D) Relative protein expression of AKT. (E) Relative protein expression of p-AKT(Ser473). (F) Relative protein expression of PI3K. (G) CCK-8 used for Ishikawa cells rescue experiment showed that OA inhibited cell proliferation, but the mixture of OA and PHTPP reversed this effect. (H) Protein expression level of ERβ when treated with DMSO, OA (10 µM), PHTPP(1 pM) and OA (10 µM) + PHTPP(1 pM). (I) Relative protein expression of ERβ when treated with DMSO, OA (10 µM), PHTPP(1 pM) and OA (10 µM) + PHTPP(1 pM). (J) Immunofluorescence analysis of subcellular localization and protein expression level of ERβ (red). Cell nuclei were stained with DAPI (blue) (Scale is 20 μm). (K) Average fluorescence of ERβ.

*p < 0.05, **p < 0.01, ***p < 0.001 vs. OA 0 µM group.

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