Fig. 3

Development of gametocytemia and transmissibility to An. stephensi mosquitoes. Female gametocyte specific transcript pfs25 (blue bars) and male pfMGET transcript density (red bars) of each participant at transmission time points (a-d). Infection intensity at oocyst stage (yellow bars) and sporozoite stage (green bars) (e–h). Baseline transmission assays were conducted on day 25 before Participants 1 and 4 were administered a single dose of 0.25 mg/kg primaquine. Participant 2 and Participant 4 were administered artemether–lumefantrine on day 32 as a rescue treatment following asexual parasite regrowth. A/L, artemether–lumefantrine; PQ, primaquine. †: no pfMGET transcripts were detected at these time points. *: sporozoite stage mosquitoes from these time points were not analysed given that no oocysts were detected.