Fig. 7

Modelling of ZIKV-induced brain tumour secretome endocrine signalling to lymph node immune cells. Diagram detailing polarisation states of lymph node immune cells following cytokine stimulation, as reported by the Immune Dictionary⁴¹. Only ZIKV-induced brain tumour cell-secreted cytokines that are dominant drivers of immune cell polarisation were assessed, as determined by the Immune Dictionary. Polarisation states, their nomenclature and the top 20 upregulated cytokine-induced DEGs (FDR ≤ 0.05) were sourced from the Immune Dictionary. Phenotypic DEG markers that indicate immune cell activity or phenotype following cytokine stimulation are listed below the respective polarised state, with colours denoting if the DEG is a cytokine (yellow), receptor (green), cytotoxicity-related gene (red), survival-related gene (blue), cell lineage marker (grey), cytokine signalling regulator (pink), or a gene indicating general immune cell activity (activation, migration or differentiation) (orange). IL-1 alpha and IL-1 beta induce the same polarisation state and are thus collectively represented as IL-1. * denotes a DEG induced by either IL-1 alpha or IL-1 beta, but not both. Abbreviations, Zika virus (ZIKV), differentially expressed gene (DEG), conventional Dendritic Cell (cDC), Natural Killer (NK), CD8+ T cell (T8), CD4+ T cell (T4), Monocyte (Mono), Macrophage (Macro), Neutrophil (Neu). Figure created in BioRender. BioRender.com/u63c617.