Table 1 Gene name, accession number, primer sequences, amplicon sizes, and optimal annealing temperatures.
From: HOXD1 regulates neural crest cells differentiation and polycerate development in sheep
Gene name | Accession number | Primer sequences (5′–3′) | Tm (°C) | Product length (bp) |
|---|---|---|---|---|
GAPDH | NC_000072.7 | F: AGGTCGGTGTGAACGGATTTG | 60 | 123 |
R: TGTAGACCATGTAGTTGAGGTCA | ||||
HOXD1 | XM_004004562.5 | F: ACGAATCGAGATCGCCAACA | 60 | 186 |
R: CTTCCAGGGTTCTTTCCGCT | ||||
TGF- β | NC_000068.8 | F: ACAGCAAAGATAACAAACTCCACG | 60 | 197 |
R: CTTCTCTGTGGAGCTGAAGCA | ||||
TGF-BR | NC_000070.7 | F: TCCTTCAAACGCGCTGACAT | 60 | 200 |
R: CAGCTCTGCCATCTGTTTGG | ||||
Samd3 | NC_000076.7 | F: TGTCAACCAGGGCTTTGAGG | 60 | 179 |
R: CCATCTGGGTGAGGACCTTG | ||||
Samd4 | NC_000080.7 | F: GGGAGGAGATCGCTTTTGCT | 60 | 98 |
R: CCAACTGCACTCCTTTGCCT | ||||
Wnt2 | NC_000072.7 | F: GGCCTGTAGCCAAGGAGAAT | 60 | 172 |
R: GGCATCCTTGCCTTTCCTCT | ||||
β-catenin | NC_000075.7 | F: CTGGGACTCTGCACAACCTT | 60 | 134 |
R: CAGTGTCGTGATGGCGTAGA | ||||
Vimentin | NC_000068.8 | F: GCTTCTCTGGCACGTCTTGA | 60 | 160 |
R: TGAGGTCAGGCTTGGAAACG | ||||
E-cadherin | NC_000074.7 | F: CAGCCGGTCTTTGAGGGATT | 60 | 128 |
R: TGACGATGGTGTAGGCGATG | ||||
α-SMA | NC_000085.7 | F: GCTACGAACTGCCTGACGG | 60 | 127 |
R: GCTGTTATAGGTGGTTTCGTGG | ||||
CXCL2 | NC_000071.7 | F: CATAGCCACTCTCAAGGGCG | 60 | 103 |
R: TCAGTTAGCCTTGCCTTTGTTC | ||||
CXCL3 | NC_000071.7 | F: TGCCTGAACACCCTACCAAG | 60 | 88 |
R: CTTCTGTCTGGGTGCAGTGG | ||||
IL-1 A | NC_000068.8 | F: ACGTCAAGCAACGGGAAGAT | 60 | 124 |
R: AAGGTGCTGATCTGGGTTGG | ||||
Mmp3 | NC_000075.7 | F: TTCTGGGCTATACGAGGGCA | 60 | 84 |
R: CTTCTTCACGGTTGCAGGGA | ||||
Chi3l1 | NC_000067.7 | F: GGAGAAGAAGCTGGCAGGAG | 60 | 105 |
R: TCCTTGATGGCGTTGGTGAG | ||||
Tgfbi | NC_000079.7 | F: TCAAAGATGGTGTCCCTCGC | 60 | 139 |
R: AAGACTCGCAGCTTTTTGCC | ||||
Prelp | NC_000067.7 | F: TTGCCCCAACAACCTAGTGG | 60 | 120 |
R: ATGAGGTCCAGTGGGATGGG | ||||
Ogn | NC_000079.7 | F: CGACCTGGAATCTGTGCCTC | 60 | 131 |
R: TTCGCTCCCGAATGTAACGA | ||||
Megf6 | NC_000070.7 | F: CTGTGATTGTGGGGGTGGG | 60 | 124 |
R: GGGTGCTGGTCTGATAGAGG | ||||
Adamts5 | NC_000082.7 | F: TGTTGCTGCTACTGCTGCT | 60 | 91 |
R: TGCCTGGTTTTATCCTGGGC |
