Fig. 2
From: Chronic starvation induces microglial cell depletion in an activity-based anorexia model
(a) Mean body weight per day normalized to body weight at habituation (days 1–7). ***p ≤ 0.001, repeated-measures ANOVA (rmANOVA) with post hoc Bonferroni correction over the complete starvation phase between control and all ABA animals (b) Mean food intake per day normalized to food intake at habituation (days 1–7). ***p ≤ 0.001, rmANOVA with post hoc Bonferroni correction over the complete starvation phase between the control and all ABA-treated animals. (c) The mean running wheel activity (RWA) normalized to the RWA at habituation (days 1–7), rmANOVA with post hoc Bonferroni correction over the complete starvation phase. #: significant changes (p = 0.018) for rmANOVA between ABA_O and ABA_V, including daily individual values; $: p = 0.076 indicates a trend toward a lower RWA in ABA_P than in ABA_V. (d) Mean running wheel activity (RWA) per phase normalized to RWA at habituation (mean ± SD), n = 59 (control = 14, ABA_V = 15, ABA_O = 15, ABA_P = 15), **p ≤ 0.01, two-way ANOVA with post hoc Bonferroni correction. Individual values from resistant animals are highlighted in red. (e) Representative image (half brain) of GFAP staining from a control animal. Enlarged images for evaluation were taken in regions of interest: the cortex cerebri (CX) and corpus callosum (CC). Control group with food ad libitum and no interventions, ABA_V ABA animals with water interventions, ABA_P ABA animals with probiotic interventions, ABA_O ABA animals with omega-3 FAs interventions.
