Table 2 Primers for real-time polymerase chain reaction, s sense; as anti-sense.
From: Chronic starvation induces microglial cell depletion in an activity-based anorexia model
Primer | Annealing temperature | Direction | Nucleotide sequence |
|---|---|---|---|
cyclophilin A | 65 °C | s | 5′-GGCAAATGCTGGACCAAACAC |
as | 5′-TTAGAGTTGTCCACAGTCGGAGATG | ||
GFAP | 61 °C | s | 5′-AGAAAACCGCATCACCATTC |
as | 5′-GCACACCTCACATCACATCC | ||
AIF1 | 65 °C | s | 5′-TGGAGTTTGATCTGAATGGCAATG |
as | 5′-AGCCACTGGACACCTCTCTA | ||
OLIG1 | 63 °C | s | 5′-CGAGCGGAAGCGCAGCAGGA |
as | 5′-AGAGCGAACTGGCCGCACG | ||
MAP2 | 58 °C | s | 5′-GCAAAGTAAGCCTGGTGA |
as | 5′-ATCTAAGGGAAGAGTGAAAC | ||
Ki-67 | 64 °C | s | 5′-CTGCAGAGAAGGTTGGGATAAA |
as | 5′-CTGACTTTGCCCAGAGATGAA | ||
APC | 65 °C | s | 5′-ATAGCCTGCCCTTGACTGAG |
as | 5′-TCTGCCGTGCTTCATACTCC |
