Fig. 4 | Scientific Reports

Fig. 4

From: HaloPROTAC3 does not trigger the degradation of the halotagged parasitophorous vacuole membrane protein UIS4 during Plasmodium liver stage development

Fig. 4

VHL-recruiting HaloPROTAC3 triggers the degradation of soluble cytosolic and transmembrane HT fusion proteins in Huh7 cells. Chemical structure of (a) the VHL control binder and (b) HaloPROTAC3. Illustration of the (c) mCherry-GFP-HT and (d) HT-GFP-FIS1 constructs expressed in Huh7 cells. Airyscan micrographs showing DNA (blue) and GFP fluorescence (green) in (e) mCherry-GFP-HT and (f) HT-GFP-FIS1 Huh7 cells. Scale bars are 10 μm. Confocal micrographs of Huh7 (g) mCherry-GFP-HT and (h) HT-GFP-FIS1 cells treated with 1 µM of the VHL binder or HaloPROTAC3. Scale bars are 50 μm. Dose-response curves showing that HaloPROTAC3 triggers degradation of (i) soluble cytosolic mCherry-GFP-HT and (j) transmembrane HT-GFP-FIS1 proteins in Huh7 cells. Average fluorescence intensities from technical duplicates were normalized and presented as means with SEMs (N = 2–3). 100% corresponds to DMSO-treated samples. GFP, green fluorescent protein; FIS1, mitochondrial fission 1 protein.

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