Table 2 qPCR thermal cycling conditions.
From: A stretchable and biomimetic polyurethane membrane for lung alveolar in vitro modelling
Cycle Step | Temperature (°C) | Time (s) | Cycles |
|---|---|---|---|
Initial Denaturation | 95 | 60 | 1 |
Denaturation | 95 | 15 | 40 |
Anneal | Variable (see Tm) | 30 | |
Extend | 72 | 60 |
