Fig. 7

Functional significance of the key gene IL6R. (A) Differential expression of IL6R in normal and tumor samples from patients with PDAC, using data from the TCGA cohort and the GTEx database. (B) Validation of IL6R expression in normal and tumor samples from local PDAC patients using qPCR. Kaplan-Meier survival curves illustrating the survival outcomes associated with low and high IL6R expression levels in (C) the TCGA cohort and the GTEx database, and (D) the ICGC cohort. (E) Heatmap representing the expression of IL6R across major cell lineages, using public databases. (F) qPCR analysis of M2-like polarization markers (YM1, MRC1, and IL10) in murine peritoneal macrophages following treatment with IL-4 and an IL-6R blocker. (G) qPCR analysis of M2-like polarization markers (YM1, MRC1, and IL10) in murine peritoneal macrophages following treatment with tumor supernatant and an IL-6R blocker. ^*P<0.05, ^**P<0.01, ^***P<0.001.