Fig. 2 | Scientific Reports

Fig. 2

From: Interleukin-7 promotes porcine early embryogenesis in vitro and inner cell mass development through PI3K/AKT pathway after parthenogenetic activation

Fig. 2

Effect of IL-7 and wortmannin (Wort) treatment during IVC for 7 days on early porcine embryonic development after parthenogenetic activation (PA). (A) Experimental design to examine the effects of IL-7 and the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway on porcine early embryogenesis and ICM development after PA. (B) Representative morphology images of cleaved embryos after Day 2 of PA and blastocysts from each group after Day 7 of PA. Scale bar, 200 μm. (C) The cleavage pattern and (D) blastocyst formation rates of the PA embryos. Embryos were treated with IL-7 (10 ng/mL) and/or Wort (1 µM). The number of cultured embryos in each group was Control (n = 197), IL-7 (n = 200), Wort (n = 195), and IL-7 + Wort (n = 199). Within each end point, bars with different letters (a-c) are significantly different (p < 0.05) among groups. For all graphs, the values represent the mean ± SEM. The experiment was independently replicated five times. Frag, Fragmentation; CL, Cleavage; BL, Blastocyst.

Back to article page