Table 2 Primers required for qRT-PCR of the four AT-DEGs.
From: To establish and validate autophagy related biomarkers for the diagnosis of IgA nephropathy
Gene | ID | Forward primer | Reverse primer | Tm |
|---|---|---|---|---|
SIRT1 | 23,411 | 5’-TGCTGGCCTAATAGAGTGGCA-3’ | 5’-CTCAGCGCCATGGAAAATGT-3’ | 49.8 |
BAG3 | 9531 | 5’-TTCCACCAAGCCCAGAAGAC-3’ | 5’-CAGGTGCAGTTTCTCGATGG-3’ | 53.8 |
CDKN1A | 1062 | 5’-GTGGGGTTATCTCTGTGTTAGGG-3’ | 5’-CCCTGTCCATAGCCTCTACTGC-3’ | 52.1 |
FOS | 2353 | 5’-AGGAGAATCCGAAGGGAAAG-3’ | 5’-CTTCTCCTCCAGCAGGTTGG-3’ | 58.4 |
