Fig. 2 | Scientific Reports

Fig. 2

From: A novel knockout mouse model to assess the impact of one-copy loss of Hnrnpk in CD4 + T cells in chronically inflamed skin as a prelude to CTCL

Fig. 2

(A) Breeding scheme used to create Hnrnpk flox/wt Cd4CreERT2 mouse. (B) Schematic diagram illustrating the Hnrnpk knockout using Tamoxifen in Hnrnpk flox/wt Cd4CreERT2 mouse. Tamoxifen was administered to mice for 5 continuous days via intraperitoneal injection (i.p.). On day 8, the spleen was collected, and CD4 + T cells were enriched. TAM: tamoxifen, i.p: intraperitoneal. (C) Representative flow cytometry plots showing CD3 + CD4 + T cells in splenocytes before and after CD4 + T cell enrichment. (D) Agarose gel electrophoresis image displaying the PCR product of recombinant Hnrnpk following Hnrnpk deletion (Hnrnpk KO) using three different sets of primers, and unrecombined Hnrnpk (Hnrnpk WT) also using three different sets of primers. (Original gel is presented in Supplementary Fig. 2).

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