Fig. 5

Plasma metabolome and lipidome of Colombian athletes with different energy demands. (A) Orthogonal partial least squares discriminant analysis (OPLS-DA) reveals significant separation between the sample groups, with notable performance metrics. (B) T-test analysis was conducted to visualize the differences in metabolite abundance between the two types of sports (WA—weightlifters and CA—cyclists). The p-value threshold for statistical significance was set at 0.05 FDR, as indicated in the (C) Hierarchical clustering heatmap from lipidomic analysis: This analysis provides a comprehensive overview of the lipidomic species identified across the blood samples of the WA and CA groups. Notably, triglycerides have a strong abundance profile in cyclists, whereas other types of lipids, such as sterols and phosphocholines, are more abundant in weightlifters. Graphics and comprehensive analysis were performed with MetaboAnalyst 6.048.