Fig. 5 | Scientific Reports

Fig. 5

From: The differential impact of HNRNPA1 isoforms on gene expression and their relevance to dsRNA-mediated innate immune response

Fig. 5

HNRNPA1 isoform ratio does not modify the levels of innate immunity genes. (A) Schematic of HNRNPA1 pre-mRNA transcript (exon 7 to 8) with location of the pro-A1B SSO. Created in BioRender. Vande Velde, C. (2025) https://BioRender.com/a65n920. (B) PCR for HNRNPA1 (primers located in exon 7 and 8) in untransfected cells and cells treated with NTC or pro-A1B SSO for 24 and 48 h at 3, 30, 100 and 200 nM. (C) Exon 7-7B-8 / exon 7–8 was calculated and plotted. Ordinary one-way ANOVA was used for statistical analysis. (D) Western blot for hnRNP A1, hnRNP A1B and β-actin in untransfected cells and cells treated with NTC or pro-A1B SSO for 24 and 48 h at 3, 30, 100 and 200 nM. (E) RT-PCR for HNRNPA1 and (F) qRT-PCR for cells treated with 30 nM of NTC or pro-A1B ASO for 24 h with and without poly (I:C). (G,H) qRT-PCR for HNRNPA1 and ISG with overexpression of myc-hnRNP A1 or myc-hnRNP A1B cDNA.

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