Fig. 7 | Scientific Reports

Fig. 7

From: Mechanotransductive stabilization of HIF-1α is inhibited by mitochondrial antioxidant therapy in the setting of pulmonary overcirculation

Fig. 7

Isolated PA rings from 4-5th generation vessels were mounted on a pressure myograph and pre-constricted with norepinephrine, then assessed for response to the endothelial and NO-dependent vasodilator acetylcholine (Ach). (A) Rings from the MitoQ shunt animals exhibited significantly improved relaxation compared to untreated shunts in response to Ach, though not at the level of normal control animals. N = 7 animals in each group. Analysis was performed by 2-way ANOVA for each dose of Ach with α of .05 as the significance threshold. * denotes significant difference between shunt and control, # between MitoQ shunt and control, and & between MitoQ shunt and shunt. Changes in mean PA pressure and pulmonary vascular resistance (PVR) were calculated in response to intravenous Ach and inhaled NO (iNO) were assessed in anesthetized and ventilated MitoQ shunt and shunt animals. (B) MitoQ shunt animals exhibit a significantly larger decrease in PVR in response to the endothelium-dependent vasodilator Ach compared to untreated shunt animals. The endothelium-independent vasodilator iNO does not demonstrate a significant decrease in PVR between groups. Data is presented as percent deviation from the immediate pre-treatment baseline. N = 10 MitoQ shunts and untreated shunts for physiologic analysis.

Back to article page