Fig. 2

ElastiGate application on a CAR-T cell manufacturing dataset. ElastiGate was used with 3 training files for all apheresis target fcs files and 1 training file for all final product fcs files. The first 5 cleanup gates were applied to all files, and the remaining gates were applied to non-Isotype fcs files. (A) The distributions of F1 scores for the first 5 gates over all 71 apheresis and final product target fcs files comparing ElastiGate to gates drawn by three manual expert analysts (Manual 1–3). Values represent the mean and error bars represent standard error of the mean (SEM). The heatmap bar represents number of cells in each gate as a Log10 scale. (B) The distributions of F1 scores for the last 7 gates, for 35 apheresis target fcs files (Isotype files are excluded). (C) The distributions of F1 scores for 3 gates, for 11 final product target fcs files (Isotype files are excluded). The % Transduction gate was calculated using the “Sample” final product fcs files. Other gates were excluded due to a small cell number, with < 30 cells. WBC: White blood cells.