Fig. 3
From: Pharmacokinetics and the effectiveness of pyrogen-free bioabsorbable wet adhesives
Phagocytosis assay of POLARIC-labeled phosphopullulan using Confocal laser scanning microscopy. (a) Synthesis of POLARIC-labeled phosphopullulan. (b) POLARIC-labeled phosphopullulan (red) was observed around the nuclei of RAW 264.7 cells (murine leukemia macrophages), which were stained with DAPI (blue). (c) POLARIC-labeled phosphopullulan (red) was observed around the nuclei of osteoclasts, which were stained with DAPI (blue). (d, e) Phagocytosis of POLARIC-labeled phosphopullulan (red) in intraperitoneal cells analyzed by immunofluorescence staining. (d) Neutrophils were stained with FITC-Gr-1 (green), a neutrophil marker, and DAPI (blue) for nuclei. Phosphopullulan (red) was rarely detected in neutrophils. (e) Macrophages were stained with FITC-F4/80 (green), a macrophage marker, and DAPI (blue) for nuclei. Phosphopullulan (red) was observed within the cytoplasm of macrophages.
