Fig. 10

The toxicity of IS20 in Danio rerio experimental model. (A) Toxicity (expressed as LC50) after 24, 48 and 72 h of incubation with IS20; (B) Toxicity (expressed as LC50) of IS20 in FET after 96 h of incubation; (C) Cardiotoxicity, measured as heart rate [beats per minute], the statistical analysis of the data was performed using a one-way ANOVA with Tukey’s post-hoc test: *p < 0.05 compared to the untreated control (E3) fish; (D) Example of malformations stated for the incubation with IS20 versus untreated control (E3) fish; PE pericardial edema, TA tail autophagy.