Fig. 6
Effects of the PRP infusion on the polarization and development of macrophages. (A, B) DEGs and GO analysis revealed that pathways which were most enriched in M1-type macrophages (top 3) were the innate immune response, the inflammatory response, and the regulation of cytokine production. (C, D) Significantly enriched pathways (top 3) in M2-type macrophages were the positive regulation of immune system processes, regulation of the immune response and lymphocyte activation. (E) GVSA scores indicated that signalling pathways associated with VEGF were predominant in paired endometrial samples (red rectangle). (F) Both the M1 and M2 phenotypes clearly shifted from phase 2 to phase 4 after PRP therapy. Diverse dot-points of the M1 and M2 phenotypes reflected the active state of the macrophages was affected by the PRP infusion. (G) Strong staining for a typical protein marker of the M1 type (iNOS), and none staining for a marker of the M2 type (Arg1) were observed after autologous PRP therapy (DAB, 50 µm). Clear staining for CD31 was also detected in various epithelial cell niches of the endometrial tissue (DAB, 50 µm).
