Fig. 3

SET/I2PP2A as a direct target of miR-494-3p. (A) Venn diagrams for potential targets of miR-494-3p. (B) Schematic diagram of 3′ UTR of SET/I2PP2A mRNA carrying potential binding site of miR-494-3p, as predicted by TargetScanHuman, version 8.0, and miRDB. (C) RT-qPCR analysis of SET/I2PP2A mRNA expression using NCI-H2030, DFCI024, and NCI-H647 cells treated with either scrambled control or miR-494-3p AS (AS). (D) Western blot analysis of SET/I2PP2A protein expression in NCI-H2030 cells following miR-494-3p AS treatment. HSP90 served as a loading control. Relative intensities of the bands were quantified using the ImageJ software. (E) Dual luciferase assay using NCI-H2030 cells treated with either scrambled control or miR-494-3p AS (AS). Columns in C and E indicate average of triplicate samples from representative experiment. Bars indicate SD. P values were calculated using unpaired t-test.