Table 7 Levels of independent variables utilized in definitive screening design for optimizing the delignification efficiency (%) of Garlic peels (30 mg mL− 1) using laccase from V. salarius.
Independent variable | Unit | Symbol | Coded variable level | ||
|---|---|---|---|---|---|
Low (− 1) | Medium (0) | High (+ 1) | |||
Concentration of HBAa | mM | A | 1 | 5.5 | 10 |
Laccase content | U L− 1 | B | 50 | 125 | 200 |
pH | – | C | 5 | 7 | 9 |
Temperature | °C | D | 30 | 40 | 50 |