Fig. 3 | Scientific Reports

Fig. 3

From: A simplified and efficient method for isolating small extracellular vesicles for comparative and comprehensive translational research

Fig. 3

Multi-omics applications of plasma-derived sEVs isolated using precipitation with ultrafiltration (CPF) method. (a) A total ion liquid chromatography-mass spectrometry (LC-MS) chromatogram of sEVs proteins with relative abundance by retention time. (b) Melt-curve analysis of miR-16-5p reveals that single peaks were seen in the melting curves at such annealing temperatures, suggesting a highly selective amplification, and (c) raw Cq values for miR-16-5p in three samples. (d) A total ion LC-MS chromatogram of sEVs lipids with relative abundance by retention time.

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