Fig. 5 | Scientific Reports

Fig. 5

From: A simplified and efficient method for isolating small extracellular vesicles for comparative and comprehensive translational research

Fig. 5

Clarification steps for biological samples and isolation of sEVs using four different protocols. (a) Unstimulated saliva; (b) plasma; (c) conditioned cell culture media samples before sEV isolation; (d) final clarification step; (e) filter with 0.22 μm syringe filters; (f) PEG-based precipitation (CP); (g) PEG-based precipitation with ultrafiltration (CPF); (h) Ultracentrifugation (UC); and (i) Size Exclusion Chromatography (SEC) columns.

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