Fig. 3
Comparison of the chemokine and cytokine secretion profile of each cell model. Cells were treated with inflammatory mediators (LPS, IL1β, IFNγ, TNFα) or vehicle (0.1% BSA in PBS) for 24 h, and conditioned media collected for measurements of analytes using the Proteome Profiler Cytokine Array Kit. Representative blots from vehicle and LPS treated iPSC-derived microglia (A1,2). For comparative analysis, each cell model’s treatment conditions were normalised back to vehicle. PCA plot displaying the clustering of each cell models treatment conditions (B). Pearson correlation matrices comparing the secretome of each cell type in response to LPS (C) IL1β (D), IFNγ (E) and TNFα (F) treatment. Scale bar denotes correlation coefficients, coloured according to value (blue, − 1.0, negatively correlated, red, 1.0, positively correlated).
