Fig. 5
Comparison of phagocytic activity across microglial models. Human microglia, iPSC-derived microglia, human pericytes, or mouse microglia were plated in 48-well plates and incubated with 1 μm FluoSpheres™ Polystyrene Microspheres for 5 min, 1 h, 2 h, or 4 h. Cells were washed thoroughly to remove uninternalised beads, collected by trypsinisation and stained with 7-AAD to allow for gating on live cells. Representative fluorescent image confirming uptake of fluorescent beads by CD45 + human microglia (A). Scale bar = 50 μm. Active phagocytosis of beads was determined by a rightward shift in FL1 intensity via flow cytometry (B). Percentage of phagocytic cells at each time point for the four cell models (C). Mean fluorescent intensity (MFI) of gated phagocytic cells for each cell model at each time point (D). n = 3 independent repeats for each cell model, data presented as mean ± SEM. Two-way ANOVA with Dunnett’s multiple comparison test comparing the percentage of phagocytic cells and MFI at each time point to that of human microglia. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001. Shared cell model key for C and D.
