Fig. 4

Inhibition of TOM7 expression attenuated mitophagy, apoptosis, mitochondrial oxidative stress and neuronal damage. (A-C) Inhibition of TOM7 expression significantly reduced the fluorescence intensity of LC3B (red) and TOMM20 (green) in the EC and DG (3 day; n = 4/group). DAPI, blue. Bar = 30 μm. (D-H) The changed levels of P62, active caspase-3 and caspase-3 in the cortex and hippocampus caused by TOM7 intervention (n = 4/group). (I-L) Mito-SOX levels in the cortex and hippocampus detected by fluorescence microplate reader and flow cytometry (n = 4/group). (M,N) Neuronal damage in the DG, CA3 and EC (3 day; n = 4/group). Bar = 50 μm. Mean ± SEM are shown. Compared with controls, *P < 0.05, **P < 0.01, and ***P < 0.001 (one-way ANOVA); and ^#P < 0.05, ^##P < 0.01, compared with each other (one-way ANOVA with Dunnett’s T3 post-hoc test). C cortex, CA3 cornu ammonis area 3, DG dentate gyrus, EC entorhinal cortex, H hippocampus, Hy Hypoxia, LC3B microtubule-associated protein light chain 3B, TOMM20 translocase of outer mitochondrial membrane 20, TOM7 translocase outer mitochondrial membrane 7.