Fig. 5
Effect of nicorandil (150 mg/kg, twice, 2 h apart, PO) on cold allodynia and the corresponding AUC induced by acetone after chronic constriction injury in rats (CCI) treated with L-arginine (A, B) (500 mg/kg, I.P, 30 min before first dose of nicorandil), methylene blue (C, D) (10 mg/kg, I.P, 30 min before first dose of nicorandil) and sildenafil (E, F) (2.5 mg/kg, I.P, 30 min before each dose of nicorandil). Cumulative score assessed by acetone test was evaluated at 1.5, 3.5, 5.5 and 7.5 h after first dose of nicorandil given on day 14 after surgery. All results were expressed as mean ± SEM (n = 5–8). Results were analyzed by two-way ANOVA in figures (A, C, E) and one-way ANOVA in figures (B, D, F) followed by Tukey’s post hoc test* P < 0.05 VS sham, + P < 0.05 VS CCI, # P < 0.05 VS CCI- nicorandil.
