Fig. 4

Antibody titers in serum (A) and skin mucus (B) obtained from European eel elvers that were immunized with immersion FCK bacterin (VACC) or those fed on diets supplied with Bacillus species probiotics (0.5 g/kg diet; PRO-F1) or (1 g/kg diet; PRO-F2), and control non-vaccinated group that fed on a basal diet (CON). Samples were taken on the 28th day of the experiment. Antibody titters were determined by the Microtiter plate agglutination test. Data were expressed as means ± SE of three biological replicates and three technical replicates per group (n = 3). The comparison between the experimental groups (VACC, PRO-F1, and PRO-F2) with the control (CON) group was conducted by one-way ANOVA analysis followed by Dunnett’s multiple comparison test.