Fig. 2
From: Efficacy and safety of cryoablation procedures using mouse models of bladder cancer
Pathological bladder changes in safety validation experiments: (A) Macroscopic findings of the bladder after cryoablation. The illustration corresponds to a macroscopic finding taken 3-h after cryoablation. The ablation site is clear, as shown in red in the illustration. POD, postoperative day. (B) Comparison of bladder muscle layer tissue in the ablation treated area and non-treated area. The illustration corresponds to an H&E-stained image of the whole bladder of 3-hours postoperatively. The area enclosed by the black dotted line indicates the ablation area. The right-side two images are enlarged views of the boxed areas in H&E-stained imaging of the whole bladder (a, ablation treated area; b, non-treated area). Scale bars in H&E-stained images indicate 100 μm (left image) and 25 μm (right images). (C) H&E-stained images of the bladder after cryoablation. Scale bars indicate 50 μm. (D) Distribution of α-SMA in the bladder muscle layer after cryoablation visualized after immunohistochemistry. Brown staining indicates α-SMA-positive cells. Scale bars indicate 50 μm. (E) Tumor shrinkage rate at each postoperative time point relative to preoperative tumor volume. A comparative test with the POD 0 group showed significant tumor shrinkage in the POD 7, 14, and 28 groups. *p < 0.0001.
