Fig. 4
From: Rapid visual detection of Treponema pallidum using the RPA-CRISPR/Cas12a system
Methodological evaluation of RPA-CRISPR/Cas12a based on fluorescence detection. (A) Real-time fluorescence curve detected from the RPA-CRISPR/Cas12a System for recombinant plasmid tp47 at different concentrations ranging from 1 fg/test to 1 ng/test (5.56 copies/ul-5.56 × 106 copies/ul). (B) Fluorescence images of eight reactions (R1-R8, R1: 1 ng/test, R2: 100 pg/test, R3: 10 pg/test, R4: 1 pg/test, R5: 100 fg/test, R6: 10 fg/test, R7: 1 fg/test, R8: NC.) were captured using a scanner. (C) Linear relationship between the fluorescence and the logarithm of target DNA concentrations. Error bars represent standard deviation, n = 3. (D) Real-time fluorescence curves detected from the RPA-CRISPR/Cas12a System for TP, HIV, HBV, HCV, and DENV. (E) Photograph (top) and bar graph depicting fluorescence intensity for TP, HIV, HBV, HCV, and DENV.
