Table 1 The oligonucleotide sequences for the primer, crRNA, and SsDNA reporter.
From: Rapid visual detection of Treponema pallidum using the RPA-CRISPR/Cas12a system
Name | Oligonucleotide sequence (5’-3’) |
|---|---|
tp47F1 | GTTCCTCATGAATTAAAAGGGATTGCAAAG |
tp47R1 | AAAAACTATCCTCAGTGAGCATTGTCTTAAGG |
tp47F2 | CGAGGAATACAAGATTACGAACGTAAAGGT |
tp47R2 | CAGAAAAACTATCCTCAGTGAGCATTGTCT |
tp47F3 | TAAGACAATGCTCACTGAGGATAGTTTTTC |
tp47R3 | ACATAGTCGATGAACTCACGGTGCGACAGC |
crRNA | UAAUUUCUACUAAGUGUAGAU UGCACGUAAGGUAAGCAGCA |
ssDNA-FQ | 6-FAM- TTATT-BHQ1 |
ssDNA-FB | 6-FAM- TTATT-Biotin |
