Fig. 4

Aberrant Zinc transporter 3 (ZnT3) immunolabeling in resected hippocampi from patients with MTLE relative to postmortem control. Micrographs show altered labeling in five resected samples (A, B, C, D, E, D1) relative to a control (F, F1). ZnT3 immunolabeling is dramatically lost in the hilus/CA3 and CA1 areas in the resected hippocampi. The inner molecular layer (iML) is apparently widened. The labeling intensity appears to be stronger in the iML, outer molecular layer (oML) and CA2 in the resected relative to control sections. The labeling in CA2 appears to extend into CA1 and remains as a band across CA1 and further entering the subicular regions. As moving from CA1 towards the Sub, this band is separated into fibrous bundles more densely located around the borders of the stratum pyramidale (s.o.), which appear to be consistent anatomically with the so-called infra- and supra-pyramidal mossy fiber bundles (IMF, SMF, pointed by arrows) (A and enlarged area, D, D1). The immunolabeling in the preserved subicular areas and temporal cortex (in a subset of samples) in the resected hippocampi appears neuropil-like (A-E), as is the labeling across CA1, Sub and the temporal cortex in the postmortem control (F, F1). The densitometric method and results are demonstrated in (G) and (H), with statistically significant intergroup difference marked by asterisks (*). Abbreviations are as defined in Fig. 3. Scale bars are as indicated.