Fig. 5

Aberrant β-secretase 1 (BACE1) immunolabeling in resected hippocampi from patients with MTLE relative to postmortem control. Panels (A-F) show low magnification images, with the framed areas enlarged as indicated (E1, E2). The mossy fiber (MF) labeling is reduced in the hilus and CA3, with the overall neuropil labeling in CA1 also reduced (with variability between cases) in the resected hippocampi (A-E) relative to control (F). The labeling in the subiculum appears to be increased in some (B, E), but reduced in other (C, D), resected hippocampi. The labeling intensity in the iML and CA2 appears to be higher in the resected hippocampi than control. Also, a band-like labeling (arrows) remains in all resected hippocampi from CA2 across CA1 and extending into the subiculum. Mossy cells in the hilus and some remaining interneurons (arrowheads) in CA1 show light BACE1 labeling (E1, E2). The remaining MF terminals in the hilus form chunk-like structures that are fairly large in size and distributed distantly in comparison with control (E1, arrows). (G) shows the methodology for measuring specific optic densities in hippocampal subregions using the white matter (WM) area as a background cutoff. (H) Graph and statistical results from one-way ANOVA analysis, with the * symbol indicating significantly intergroup difference. Scale bars are as indicated.