Abstract
This study assessed the feasibility of posterior mitral valve leaflet and subvalvular reconstruction in an acute porcine model using a novel 2-ply vacuum-pressed small intestinal submucosal extracellular matrix (SIS-ECM) patch. Reconstruction was performed in an acute 80-kg porcine model (n = 7), using a novel 2-ply vacuum-pressed SIS-ECM (CorMatrix) patch for posterior mitral reconstruction. Echocardiography and left ventricular pressure were assessed pre- and post-interventional. Sonomicrometry was used to evaluate the geometry and valve dynamics. The reconstructed mitral valves were all fully competent. The peak left atrial pressure at baseline and post-reconstruction was 9.9 (1.1) vs 9.9 (1.0) mmHg, p = 0.676, diff = 0.002 mmHg, 95% CI: [-1.06;1.05], and the mean trans-mitral pressure difference was 4.5 (2.3) vs 4.1 (2.3) mmHg, p = 0.063, diff = -0.40, 95% CI: [-2,73;1.93] The out-of-plane characteristics and subvalvular geometry of the mitral valve were preserved after reconstruction. Slight atrial bending of the reconstructed posterior leaflet and systolic annular ballooning were observed. In an acute porcine model, we successfully reconstructed the posterior mitral valve and subvalvular apparatus using our modified 2-ply vacuum-pressed SIS-ECM patch. The bioscaffold demonstrated short-term durability in vivo, warranting further investigation of its long-term durability.
Data availability
The data used in the current study are available from the corresponding author upon reasonable request.
Abbreviations
- MV:
-
Mitral valve
- SD:
-
Standard deviation
- SIS-ECM:
-
Small intestinal submucosal extracellular matrix (CorMatrix)
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Funding
This work was supported by the Novo Nordisk Foundation [Grant number NNF20OC0065584].
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JHJ, JMH, and MJT conceived the study idea, designed the study, and planned the experimental setup and protocol. JHJ and MJT designed the patch. JHJ, FTA, AEK, DOA and JTV performed the intervention and collected the data. SNS designed software for data collection and analysis. JHJ and AEK analysed and interpreted the results of the study. JHJ wrote the initial draft of the manuscript. All authors discussed the results, reviewed and revised the manuscript, and approved the final version.
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No human subjects were included in the study. All institutional and national guidelines for the care and use of laboratory animals were followed and approved by the appropriate institutional committees (nr. 2016-15-01201-01132).
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Jedrzejczyk, J.H., Andersen, F.T., Kaspersen, A.E. et al. Functional competency of a novel 2-ply vacuum-pressed biological scaffold for entire posterior mitral valve reconstruction. Sci Rep (2026). https://doi.org/10.1038/s41598-026-36236-4
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DOI: https://doi.org/10.1038/s41598-026-36236-4
