Fig. 8

Effects of the dsRNA treatments on TuMV-Svr-infected plants. (A) Disease symptoms in TuMV-Svr-inoculated N. benthamiana plants treated with naked dsRNA and the nanocomposites at 25 dpi. The treatments were as follows: Mock (treated with DEPC-treated water); dsRNA (treated with naked dsCP-HCPro); dsRNA: CDs (treated with dsCP-HCPro-loaded CDs); dsRNA: PMSNs (treated with the dsCP-HCPro-loaded PMSNs). (B) Chlorophyll content in TuMV-inoculated plants in different treatments compared with non-inoculated plants. Determinations were made at 46 and 66 dpi. (C) TuMV accumulation among the treatments with naked and dsCP-HCPro-loaded NPs determined at 7 and 37 dpi. *P < 0.05, **P < 0.01 and ***P < 0.005 represent significance after one-way ANOVA test. The effect of the treatment with naked dsCP/HC-Pro at 37 dpi resulted non-significant (P = 0.062). Bars represent mean ± SE.