Fig. 2
From: Role of MNX1-mediated histone modifications and PBX gene family in MNX1-induced leukemogenesis
Pbx family genes are upregulated in MNX1-driven pathology. Box-and-whisker plots show median, interquartile range, and full data range of (a) Relative expression of Pbx1, and (b) Pbx4 and Pbxip1, normalized to Hprt and measured by qPCR in MNX1-transduced fetal-liver cells versus empty-vector control (Ctrl), and in leukemic bone-marrow cells from MNX1-transplanted mice versus controls. (c) DESeq2-normalized expression of PBX4 and PBXIP1 in pediatric AML patient samples carrying t(7;12) compared with normal BM from healthy donors (TARGET AML dataset; Supplementary Table S8). (d) MNX1 enrichment, and (e) H3K4me3 and H3K27me3 enrichment, at the Pbx1 promoter in FL cells or leukemic BM cells, determined by ChIP-qPCR. Statistical tests: Student’s t-test (a, b, d, e) or Mann–Whitney U-test (c). Significance is indicated as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). n denotes the number of biological replicates.
