Fig. 2

Supplementation with DEIX inhibits osteoclastic activation in the inflamed periodontium of rat groups.
(A) TRAP staining images showing the ligature-mediated osteoclast formation and its suppression by DEIX (Bar = 100 μm), in which the tetragonal regions in the upper panels were 4-fold magnified. (B) The TRAP-positive area (%) in the magnified area of rat groups (n = 4). (C) IF assay images indicating the expression levels of RANKL (green) and cathepsin K (red) in the inflamed periodontium of rat groups (Bar = 50 μm). The intensities (a.u.) of (D) RANKL- and (E) cathepsin K-specific immunofluorescences were determined using the ImageJ program (n = 4). *p < 0.05 by unpaired non-parametric test (Kolmogorov-Smirnov test). ns, not significant.