Fig. 5 | Scientific Reports

Fig. 5

From: Therapeutic potency and the related mechanism of deinoxanthin in experimental animal and cell models of periodontitis

Fig. 5The alternative text for this image may have been generated using AI.

The addition of DEIX inhibits the expression of inflammation-related molecules while restoring the induction of antioxidant molecules in LPS-exposed hPDLCs. (A) Western blot images showing the immunoreactive bands of TLR4, COX-2, TNF-α, Nrf2, and HO-1 in the control hPDLCs or the cells exposed to LPS, DEIX, or both for 24 h. Original blots are presented in Figure S5. (B) Densitometric analysis results show the protein levels’ ratio to that of β-actin (n = 4). (C) The RT-PCR results showing the relative expression levels of IL-8, COX-2, Nrf2, and HO-1 in hPDLCs exposed to LPS and/or DEIX for 24 h (n = 4). (D) Western blot images exhibiting the immunoreactive bands of Nrf2 and HO-1 in the hPDLCs exposed or not to LPS, DEIX, and/or ML385 for 24 h. Original blots are presented in Figure S6. (E) The densitometric assay results showing the ratio of Nrf2 and HO-1 to that of β-actin in the cells (n = 4). *p < 0.05 by an unpaired non-parametric test (Kolmogorov-Smirnov test). ns, not significant.

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