Fig. 5 | Scientific Reports

Fig. 5

From: Construction and initial validation of key gene network for progesterone resistance in endometrial cancer based on genome-wide CRISPR screening

Fig. 5

Changes in sensitivity of Ish-MR to MPA after interference with drug-resistant genes. (A) Following the knockdown of ABCC2 expression using various siRNA sequences and subsequent rescue overexpression, Ish-MR cells were assessed for MPA drug concentration inhibition curves and apoptosis detection via flow cytometry. The IC50 values were determined to be 138.6, 96.9, 96.3, 135.0, and 167.18 µM, respectively. (B) Similar experimental interventions on AMPD3. The IC50 values were determined to be 130.8, 88.7, 76.4, 139.1, and 183.5 µM, respectively. (C) Similar experimental interventions on NNMT. The IC50 values were determined to be 119.2, 66.5, 87.8, 145.1, and 120.8 µM, respectively. (D) Similar experimental interventions on DTX3L. The IC50 values were determined to be 119.9, 87.5, 88.0, 128.0, and 142.1 µM, respectively. (E) Similar experimental interventions on PDE1A. The IC50 values were determined to be 136.4, 91.5, 80.8, 124.8, and 123.6 µM, respectively. (The IC50 value was calculated from three independent biological replicates (n = 3) using the “Quest Graph™ IC50 Calculator” (AAT Bioquest, Inc., https://www.aatbio.com/tools/ic50-calculator). The apoptosis data are presented as the mean ± SD of three independent biological replicates (n = 3). Error bar refers to mean and SD. *P < 0.05.)

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