Table 1 Statistical data of sequencing quality and sample comparison information.

From: Construction and initial validation of key gene network for progesterone resistance in endometrial cancer based on genome-wide CRISPR screening

SampleID

Baseline

MPA treatment

Control

Sequencing quality

Raw reads

25,240,962

26,167,956

25,710,382

Raw bases(G)

7.57

7.85

7.71

Clean reads

24,046,198

24,821,110

24,255,587

Clean bases(G)

0.48

0.5

0.49

Effective rate(%)

95.27

94.85

94.34

Q20(%)

98.66

98.56

98.45

Q30(%)

95.98

95.69

95.41

Error rate(%)

0.02

0.02

0.02

GC ration(%)

54.44

54.21

54.16

Comparison information

Read

24,046,198

24,821,110

24,255,587

Mappedreads

22,527,777(93.69%)

23,162,120(93.32%)

22,556,693(93.00%)

Grna_mean_depth

396

407

396

Max_depth

17,016

20,072

17,846

Median_depth

369

378

366

Totalgrnas

56,869

56,869

56,869

Zerogrnas

4

5

6

Coverage_rate

99.99%

99.99%

99.99%

Totalgenes

19,834

19,834

19,834

Zerogenes

0

0

1

Giniindex

0.05885

0.05838

0.06206

  1. Raw_reads: Raw number of reads; Raw_base(G): Raw base number; Clean_reads: Read counts after filtration; Clean_base(G): Base counts after filtration; Effective rate(%): The ratio of clean reads to raw reads; Q20(%): Proportion of the total bases with a sequencing quality value greater than 20(Generally above 90%); Q30(%): Proportion of the total bases with a sequencing quality value greater than 30(Generally above 80%); Error rate(%): Average error rate for all bases; GC ratio(%): The total content of GC bases; Read: gRNA read counts; MappedReads: Full Alignment of Reads to the gRNA Library; Grna_mean_depth: Average Sequencing Depth of gRNAs, calculated as the total number of gRNA reads aligned to the reference sequence divided by the number of gRNAs in the aligned gRNA Library; Max_depth: Maximum Sequencing Depth of gRNAs, referring to the number of alignments for the gRNA in the gRNA Library with the highest number of reads aligned among all gRNAs; Median_depth: Median Sequencing Depth of gRNAs; Totalgrnas: Total number of gRNAs in the gRNA library; Zerogrnas: Number of missing gRNAs in the gRNA library; Coverage_rate: Library coverage, defined as the proportion of detected gRNAs out of the total gRNAs(For library quality control, a coverage of > 90% is typically required); Totalgenes: Total number of genes targeted by the gRNA library; Zerogenes: Number of genes that were not detected; GiniIndex: Average abundance of gRNAs, with values between 0 and 1 (Average abundance of gRNAs, with values between 0 and 1. A smaller value indicates a more uniform distribution of sequencing reads across gRNAs, typically less than 0.1).
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