Fig. 7
From: YTHDC1 modulates the malignant phenotype of retinoblastoma via SQSTM1-mediated autophagy
YTHDC1 regulates autophagic flux in RB cells. (A and B) Western blot (A) and quantification (B) of LC3B protein expression in YTHDC1-knockdown RB cells with or without CQ treatment for 24 hours. (C) Representative images of mCherry-GFP-LC3 fluorescence in YTHDC1-knockdown RB cells. Scale bar: 5 µm. (D) Quantification of red-only (autolysosomes) in mCherry-GFP-LC3 fluorescence images of RB cells. (E and F) Western blot (E) and quantification (F) of LC3B protein expression in YTHDC1-overexpressing RB cells with or without CQ treatment for 24 hours. (G) Representative images of mCherry-GFP-LC3 fluorescence in YTHDC1-overexpressing RB cells. Scale bar: 5 µm. (H) Quantification of yellow autophagosomes in mCherry-GFP-LC3 fluorescence images. Data are mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.
